UV spectrophotometric method has been developed and validated for simultaneous estimation of tolperisone hydrochloride and aceclofenac in synthetic mixture by absorbance ratio method. Absorbance ratio method involves formation of Q-absorbance equation at 239 nm (isoabsorptive point) and also at 275 nm (λmax of aceclofenac). The different analytical parameters were determined according to ICH Q2 (R1) guideline. The method was found to be linear between the range of 3-15 µg/ml for tolperisone hydrochloride and 2-10 µg/ml for aceclofenac using methanol as solvent. The mean percentage recovery was found to be 100.255% and 99.865% for tolperisone hydrochloride and aceclofenac respectively, at three different levels of standard additions. The precision of method was found within the limits (RSD < 2%). It could be concluded from the results obtained in the present investigation for simultaneous estimation of tolperisone hydrochloride and aceclofenac in synthetic mixture are simple, rapid, accurate, precise, economical and robust.
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